Open AccessIdentification of ColE1 DNA sequences that direct single strand-to-double strand conversion by a phi X174 type mechanism.
Author(s) -
Nobuo Nomura,
Robert L. Low,
Dan S. Ray
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.10.3153
Subject(s) - dnag , dna , microbiology and biotechnology , biology , cole1 , dnab helicase , okazaki fragments , in vitro recombination , dna polymerase ii , d loop , dna replication , plasmid , dna clamp , genetics , gene , helicase , circular bacterial chromosome , eukaryotic dna replication , molecular cloning , peptide sequence , mitochondrial dna , polymerase chain reaction , rna , reverse transcriptase
A DNA single-strand initiation sequence, named rriA (called rri-1 previously), was detected in the origin region (Hae II fragment E) of the ColE1 plasmid [Nomura, N. & Ray, D. S. (1980) Proc. Natl. Acad. Sci. USA 77, 6566-6570]. Another site, called rriB, has been found on the opposite strand of Hae II fragment C. Both rriA and rriB (i) direct conversion of chimeric M13 phage single-stranded DNA to parental replicative form DNA in vivo by a rifampicin-resistant mechanism that is dependent on the dnaG and dnaB gene products, (ii) provide effector sites of dATP hydrolysis by primosomal protein n', and (iii) require the same primosomal proteins as phi X174 DNA for directing the in vitro conversion that rriA is the DNA sequence that determines the mechanism of lagging strand synthesis of ColE1 DNA and that the mechanism of discontinuous synthesis involves the primosomal proteins utilized in the in vitro conversion of phi X174 single strands to the double-stranded replicative form.