Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors. | Zendy

Stanton B. Gelvin | Zendy; Michael F. Thomashow | Zendy; James C. McPherson | Zendy; M P Gordon | Zendy; Eugene W. Nester | Zendy

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Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.

Author(s) -

Stanton B. Gelvin,

Michael F. Thomashow,

James C. McPherson,

M P Gordon,

Eugene W. Nester

Publication year - 1982

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.79.1.76

Subject(s) - octopine , plasmid , biology , microbiology and biotechnology , dna , ti plasmid , genetics , rna , southern blot , agrobacterium tumefaciens , gene , transgene

The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors were determined by blot hybridization. At least six discrete polyadenylylated RNAs (sizes 0.73-1.75 kilobases) were detected in octopine-producing tumors (lines A6S/2, E9, and 15955/01). In one tumor (line 15955/1) in which octopine could not be detected, one of the RNA species was missing and another was diminished in abundance. Evidence is presented suggesting that the transcripts are encoded entirely by the plasmid DNA and do not originate from the surrounding plant DNA sequences.

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