Thiamine as an integral component of brain synaptosomal membranes. | Zendy

Tomoki Matsuda | Zendy; J. R. Cooper | Zendy

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Thiamine as an integral component of brain synaptosomal membranes.

Author(s) -

Tomoki Matsuda,

J. R. Cooper

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.9.5886

Subject(s) - thiamine , membrane , chemistry , synaptosome , chromatography , synaptic membrane , biochemistry , cerebral cortex , thiamine deficiency , sucrose , osmotic shock , centrifugation , biophysics , biology , endocrinology , gene

Synaptic plasma membranes were prepared from rat cerebral cortex to determine if thiamine was localized in the membranes. The synaptosomes, prepared by discontinuous sucrose gradient centrifugation, were subjected to osmotic shock at pH 9.5 for 10--15 min and subfractionated on a discontinuous sucrose gradient. The two membrane fractions that were obtained were free of contamination by mitochondrial membrane and soluble fractions. In order to ensure specificity, thiamine was assayed fluorometrically before and after the addition of thiaminase I (thiamin:base 2-methyl-4-aminopyrimidine-5-methenyltransferase, EC 2.5.1.2). The thiamine content of the two membrane fractions was 9 and 10 pmol/mg protein.

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