Pleiotropic expression of Epstein--Barr virus DNA in human epithelial cells. | Zendy

Jay Stoerker | Zendy; Deborah S. Parris | Zendy; Yoshihiro Yajima | Zendy; Ronald Glaser | Zendy

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Pleiotropic expression of Epstein--Barr virus DNA in human epithelial cells.

Author(s) -

Jay Stoerker,

Deborah S. Parris,

Yoshihiro Yajima,

Ronald Glaser

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.9.5852

Subject(s) - transfection , virus , lytic cycle , epstein–barr virus , biology , dna , microbiology and biotechnology , virology , capsid , cell culture , antigen , recombinant dna , gene , genetics

We have attempted to establish a system that can be used to study the association of Epstein--Barr virus (EBV) with epithelial cells. Attempts were made to transfect human carcinoma cells with EBV DNA. Successful transfection was confirmed by the expression of EBV-specific early antigen (EA), virus capsid antigen, and the presence of virus DNA. The transfecting preparation contained a mixture of EBV and cellular DNA extracted from two producer cell lines, P3HR-1 and AG-876. Our data suggest that virus DNA obtained from the P3HR-1 nontransforming, EA-inducing strain of EBV was lytically expressed in the epithelial tumor cells. The DNA derived from AG-876 cells, which produce a transforming, non-EA-inducing strain of EBV, also produced a lytic infection.

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