Open AccessCloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.
Author(s) -
Reiner Gentz,
Annette Langner,
Annie Chang,
Stanley N. Cohen,
Hermann Bujard
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.8.4936
Subject(s) - promoter , cloning (programming) , transcription (linguistics) , biology , computational biology , genetics , microbiology and biotechnology , gene expression , computer science , gene , linguistics , philosophy , programming language
Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.