Open AccessConversion of B DNA between solution and fiber conformations.
Author(s) -
Marshal Mandelkern,
Nanibhushan Dattagupta,
Donald M. Crothers
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.7.4294
Subject(s) - dna , monomer , salt (chemistry) , chemistry , molecule , ion , crystallography , fiber , biophysics , biochemistry , organic chemistry , polymer , biology
At low salt and high DNA concentration, rodlike DNA molecules form specific aggregates containing about seven parallel DNA molecules packed in a bundle. Bundle formation can be reversed by addition of small amounts of EDTA, and formation of the specific aggregates can be induced at higher salt concentration by addition of trivalent ions. In contrast to monomeric DNA, the aggregated species has the optical anisotropy expected for the B form as observed in fibers.