Open AccessHeat shock proteins of higher plants
Author(s) -
Joe L. Key,
Ching Yu Lin,
Y. M. Chen
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.6.3526
Subject(s) - heat shock protein , protein biosynthesis , shock (circulatory) , thermal shock , biophysics , heat shock , biology , microbiology and biotechnology , in vitro , translation (biology) , biochemistry , chemistry , messenger rna , thermodynamics , gene , medicine , physics
The pattern of protein synthesis changes rapidly and dramatically when the growth temperature of soybean seedling tissue is increased from 28°C (normal) to about 40°C (heat shock). The synthesis of normal proteins is greatly decreased and a new set of proteins, “heat shock proteins,” is induced. The heat shock proteins of soybean consist of 10 new bands on one-dimensional NaDodSO4 gels; a more complex pattern is observed on two-dimensional gels. When the tissue is returned to 28°C after 4 hr at 40°C, there is progressive decline in the synthesis of heat shock proteins and reappearance of a normal pattern of synthesis by 3 or 4 hr.In vitro translation of poly(A)+ RNAs isolated from tissues grown at 28 and 40°C shows that the heat shock proteins are translated from a new set of mRNAs induced at 40°C; furthermore, the abundant class mRNAs for many of the normal proteins persist even though they are translated weakly (or not at all)in vivo at 40 or 42.5°C. The heat shock response in soybean appears similar to the much-studied heat shock phenomenon inDrosophila .