Open AccessInducibility of spleen focus-forming virus by BrdUrd is controlled by the differentiated state of the cell.
Author(s) -
I. GreiserWilke,
Wolfram Ostertag,
Peter S. Goldfarb,
Angelika Lang,
Mitsuru Furusawa,
J F Conscience
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.5.2995
Subject(s) - biology , friend virus , spleen , virus , thymidine kinase , microbiology and biotechnology , somatic cell , murine leukemia virus , haematopoiesis , thymidine , cellular differentiation , virology , biochemistry , immunology , in vitro , stem cell , gene , herpes simplex virus
All Friend cells--except thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)-deficient mutants--are highly inducible for the release of biologically active spleen focus-forming virus (SFFV) after exposure to BrdUrd. We studied SFFV production in somatic cell hybrids made between Friend leukemia cells (FLC) and cells expressing various differentiation programs. High inducibility of SFFV and release of constitutive Friend virus (FV) and SFFV are eliminated in all hybrids in which the potential for erythroid differentiation is suppressed. FV release and its induction by BrdUrd are unchanged in hybrids that maintain the expression of erythroid differentiation.