Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. | Zendy

Keith C. Robbins | Zendy; S G Devare | Zendy; S A Aaronson | Zendy

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Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones.

Author(s) -

Keith C. Robbins,

S G Devare,

S A Aaronson

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.5.2918

Subject(s) - biology , helper virus , restriction enzyme , virology , virus , plasmid , recombinant dna , microbiology and biotechnology , molecular cloning , genome , retrovirus , recombinant virus , dna , viral transformation , restriction map , gene , genetics , viral replication , peptide sequence

The integrated form of simian sarcoma virus (SSV) was molecularly cloned in the Charon 16A strain of bacteriophage lambda. In transfection analysis, the recombinant viral DNAs demonstrated the ability to transform cells in tissue culture at high efficiency. Such transformants possessed typical SSV morphology, expressed simian sarcoma associated virus (SSAV) gag gene products in the absence of virus release, and released SSV after superinfection with a type C helper virus. A physical map of the 5.8-kilobase-pair (kbp) recombinant viral DNA clone, deduced from restriction endonuclease analysis, revealed a 5.1-kbp SSV genome containing 0.55-kbp-long terminal repeats flanked by 0.45 and 0.25 kbp of contiguous host cell sequences. By R-loop analysis, the viral DNA molecule contained two regions of homology to SSAV, separated by a 1.0-kbp nonhomologous region. This SSV-specific sequence was shown to be uniquely represented within the normal cellular DNA of diverse mammalian species, including human. Our results demonstrate that this primate transforming retrovirus arose in nature by recombination of a type C helper virus and a host cellular gene.

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