Open AccessErythrosomes: large proteoliposomes derived from crosslinked human erythrocyte cytoskeletons and exogenous lipid.
Author(s) -
John Cuppoletti,
E. Mayhew,
Carsten Zobel,
Chan Y. Jung
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.5.2786
Subject(s) - liposome , chemistry , phospholipid , mannitol , phosphatidylcholine , bovine serum albumin , lipid bilayer , sodium , sucrose , biophysics , chromatography , membrane , biochemistry , organic chemistry , biology
Large (3-micrometers diameter) mechanically stable proteoliposomes (erythrosomes) were prepared in good yield by coating crosslinked erythrocyte cytoskeletons with phosphatidylcholine. The erythrosomes consist of the polypeptides designated band 1, 2, 3, 4.1 + 4.2, and 5 (less than 4% of the endogenous lipid) and enough added lipid to form a bilayer coating the surface. Electron microscopy shows only the large proteoliposomes in sealed preparations. The trapping of bovine serum albumin, mannitol, sucrose, glucose, cytosine arabinoside, and sodium in the erythrosomes was demonstrated, yielding an apparent volume of up to 100 liters/mol of phospholipid. This preparation possesses an effective diffusion barrier to glucose, sucrose, and sodium ion with half-equilibration times of 34, 29, and 170 hr, respectively. The results of the present study suggest that erythrosomes may be useful for membrane transport protein reconstitution and encapsulation systems.