Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector | Zendy

A Moriarty | Zendy; Bill H. Hoyer | Zendy; J. WaiKuo Shih | Zendy; John L. Gerin | Zendy; Dean H. Hamer | Zendy

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Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector

Author(s) -

A Moriarty,

Bill H. Hoyer,

J. WaiKuo Shih,

John L. Gerin,

Dean H. Hamer

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.4.2606

Subject(s) - virology , recombinant dna , antigen , virus , simian , hepatitis b virus , biology , viral transformation , hepatitis b virus dna polymerase , gene , cell culture , microbiology and biotechnology , immunology , biochemistry , genetics

We have constructed a simian virus 40 recombinant carrying a fragment of DNA from hepatitis B virus. Cultured monkey kidney cells infected with this recombinant produce hepatitis B surface antigen. The antigen is excreted into the culture medium as 22-nm particles with the same physical properties, antigenic composition, and constituent polypeptides as those found in the sera of patients with type B hepatitis.

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