The nucleotide sequence surrounding the replication terminus of R6K. | Zendy

Deepak Bastia | Zendy; Joseph Germino | Zendy; Jorge H. Crosa | Zendy; Jeffrey L. Ram | Zendy

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The nucleotide sequence surrounding the replication terminus of R6K.

Author(s) -

Deepak Bastia,

Joseph Germino,

Jorge H. Crosa,

Jeffrey L. Ram

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.4.2095

Subject(s) - ter protein , dna replication , pbr322 , plasmid , control of chromosome duplication , dna , eukaryotic dna replication , biology , origin recognition complex , origin of replication , replication factor c , recombinant dna , microbiology and biotechnology , sequence (biology) , base pair , c terminus , in vitro recombination , nucleic acid sequence , genetics , peptide sequence , molecular cloning , gene , amino acid

The replication terminus of the plasmid R6K has been cloned into the single-stranded DNA phage vector M13mp5 and also into the plasmid vectors pBR313 and PBR322. By using single-stranded DNA templates prepared from the recombinant DNA clones, the sequence of 215 base pairs of DNA containing the replication terminus has been determined. The DNA sequence of the region of the terminus does not contain any 2-fold rotational symmetry. Therefore, folding of the DNA at the region of the terminus is unlikely to be a cause for replication termination. Interaction of a host-specified protein(s) with the sequence of the replication terminus is probably the basis of the mechanism of replicaion termination.

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