Open AccessIdentification and purification of the Lon + (capR + ) gene product, a DNA-binding protein
Author(s) -
Barbara A. Zehnbauer,
Edward Foley,
G W Henderson,
Alvin Markovitz
Publication year - 1981
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.4.2043
Subject(s) - dna , gene , microbiology and biotechnology , biology , chemistry , genetics
The polypeptide product of thelon (capR ) gene was identified and partially purified from bacterial strains homozygous for thecapR + orcapR9 (ochre mutation) alleles cloned with pSC101. A 94,000-dalton polypeptide was identified as the lon (capR) gene product. Studies of binding to DNA cellulose columns and nitrocellulose filters indicate that the capR+ and capR9 proteins bind DNA.
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