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Spleen cells from NFR/N mice primed with carrier rabbit erythrocytes were passed through nylon wool and fused with cells from the AKR T-cell lymphoma BW5147 in order to construct functional T-cell hybridomas. Several hybrids have been obtained. One of the cloned hybrids possesses a marked capacity to functionally complement the T-cell-dependent hapten-specific plaque-forming cell responses of NFR/N nude mice to the trinitrophenyl group. The hybrids carry the Thy 1 marker of the immune parent, express the Lyt 1 surface marker, possess Fc receptors at their surface, and express an Ig heavy-chain variable region determinant at their surfaces as detected by anti-VH MOPC 315 antiserum.

Complementation of the plaque-forming cell responses of T-cell-deficient nude mice by a T-cell hybridoma.