Open AccessConstruction of recombinant plasmids containing Xenopus immunoglobulin heavy chain DNA sequences.
Author(s) -
Ronald D. Brown,
Richard W. Armentrout,
Mark D. Cochran,
Joe Cappello,
Suzanne O. Langemeier
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.3.1755
Subject(s) - microbiology and biotechnology , recombinant dna , biology , complementary dna , plasmid , xenopus , messenger rna , dna , immunoglobulin heavy chain , nucleic acid thermodynamics , rna , molecular cloning , antibody , gene , genetics
A recombinant cDNA plasmid containing Xenopus immunoglobulin heavy chain sequence has been constructed from Xenopus spleen poly(A)-containing RNA. The plasmid was identified by colony hybridization and a hybridization-translation assay and its identity was confirmed by DNA sequence analysis. The portion of the heavy chain sequence contained in the plasmid is 35% homologous to mammalian mu and gamma sequences. The mRNA corresponding to this plasmid is 2.5 kilobases, in close agreement with the size of mouse mu mRNA. RNA sequences complementary to the cloned sequence appear in embryos about 24 hr after fertilization, which corresponds to 24 hr before the first detectable immunoglobulin.