Open AccessPassage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane.
Author(s) -
John E. Bergmann,
K. T. Tokuyasu,
S. J. Singer
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.3.1746
Subject(s) - vesicular stomatitis virus , golgi apparatus , endoplasmic reticulum , immunoelectron microscopy , chinese hamster ovary cell , glycoprotein , microbiology and biotechnology , biology , transmembrane protein , virus , vesicular stomatitis , chemistry , virology , biochemistry , antibody , receptor , immunology
The intracellular pathway of biogenesis of the vesicular stomatitis virus transmembrane glycoprotein was investigated in situ by using indirect immunofluorescence of whole infected Chinese hamster ovary cells and immunoelectron microscopy of ultrathin frozen sections of infected cells. Transport of the glycoprotein was synchronized by using the temperature-sensitive virus mutant Orsay-45 and a temperature shift-down protocol. Sequential appearance of the glycoprotein in the rough endoplasmic reticulum, Golgi apparatus, and plasmalemma was demonstrated. The potential of this system for further studies is discussed.