Open AccessA small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
Author(s) -
V. W. Yang,
Michael R. Lerner,
Joan A. Steitz,
S. J. Flint
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.3.1371
Subject(s) - ribonucleoprotein , rna splicing , small nuclear ribonucleoprotein , rna , microbiology and biotechnology , biology , small nuclear rna , micrococcal nuclease , transcription (linguistics) , nuclease , non coding rna , virology , dna , genetics , chromatin , gene , linguistics , philosophy , nucleosome
The size and structure of viral RNA species synthesized in nuclei isolated during the early phase of productive infection by adenovirus type 2 have been examined by electrophoresis in denaturing polyacrylamide cells and the nuclease S1 assay. The major products of transcription in vitro of early regions 1 and 2 in the adenoviral genome are processed RNA molecules that appear to be correctly spliced in isolated nuclei. Splicing of adenoviral RNA molecules is inhibited when nuclei are preincubated with antibodies from systemic lupus erythematosus patients that immunoprecipitate small nuclear ribonucleoprotein particles. The specificity of these antibodies suggests that ribonucleoprotein particles containing U1 RNA are required for splicing of the adenoviral RNA sequences we have examined.