Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli. | Zendy

Jacques Laval | Zendy; J Pierre | Zendy; Françoise Laval | Zendy

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Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.

Author(s) -

Jacques Laval,

J Pierre,

Françoise Laval

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.2.852

Subject(s) - micrococcus luteus , depurination , escherichia coli , dna glycosylase , biochemistry , chemistry , dna , microbiology and biotechnology , biology , dna repair , gene

Cell extracts from Micrococcus luteus release both free 3-methyladenine and free 7-methylguanine from alkylated DNA. The glycosylase activity responsible for the liberation of 7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not liberate it. Furthermore, the heat inactivation rates of the two enzymatic activities are different. The release of 7-methylguanine by chemical depurination of ethanol-soluble oligonucleotides has been ruled out. A similar activity releasing 7-methylguanine is also found in Escherichia coli.

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