Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/hydrogen peroxide-generated hydroxyl radical. | Zendy

John E. Repine | Zendy; Oswald Pfenninger | Zendy; David W. Talmage | Zendy; E. Berger | Zendy; David E. Pettijohn | Zendy

Open Access

Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/hydrogen peroxide-generated hydroxyl radical.

Author(s) -

John E. Repine,

Oswald Pfenninger,

David W. Talmage,

E. Berger,

David E. Pettijohn

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.2.1001

Subject(s) - hydroxyl radical , dimethyl sulfoxide , hydrogen peroxide , dna , chemistry , scavenger , ionizing radiation , sulfoxide , irradiation , photochemistry , dna damage , radical , biophysics , biochemistry , organic chemistry , biology , physics , nuclear physics

Eighty percent of the single-strand DNA breaks induced by gamma-irradiation were prevented by the hydroxyl radical (.OH) scavenger dimethyl sulfoxide (Me2SO); CH4 was generated in the process as a product of the interaction of .OH and Me2SO. In contrast, Me2SO completely blocked DNA nicking by an iron/H2O2 system which produces .OH but smaller amounts of CH4 from Me2SO. Because Me2SO prevented DNA breaks from the more efficient iron/H2O2 system but only blocked 80% of irradiation-mediated nicking, the results suggest that .OH is responsible for 80% of the DNA single-strand breaks and the remaining 20% is due to interactions not involving .OH.

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