Spin trapping of free radicals during hepatic microsomal lipid peroxidation. | Zendy

Gerald M. Rosen | Zendy; Elmer J. Rauckman | Zendy

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Spin trapping of free radicals during hepatic microsomal lipid peroxidation.

Author(s) -

Gerald M. Rosen,

Elmer J. Rauckman

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.12.7346

Subject(s) - spin trapping , radical , microsome , lipid peroxidation , chemistry , microsoma , photochemistry , superoxide , biochemistry , antioxidant , enzyme

We have used spin-trapping techniques to identify radical species formed during the NADPH-stimulated peroxidation of rat hepatic microsomes. Using 5,5-dimethyl-1-pyrroline-1-oxide, we have confirmed the presence of substantial quantities of superoxide but found evidence for the formation of only small quantities of hydroxyl radical. Use of the spin traps N-tert-butyl-alpha-4-pyridylnitrone-1-oxide and 2-methyl-2-nitroso-1-propanol have allowed us to determine that lipid peroxyl radicals are the predominant "lipid-type" radical found in peroxidizing microsomes under aerobic conditions. These data suggest that microsomal lipid dienyl radicals react with molecular oxygen at near diffusion-controlled rates.

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