Growth of purified astrocytes in a chemically defined medium. | Zendy

Richard S. Morrison | Zendy; Jean de Vellis | Zendy

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Growth of purified astrocytes in a chemically defined medium.

Author(s) -

Richard S. Morrison,

Jean de Vellis

Publication year - 1981

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.78.11.7205

Subject(s) - astrocyte , glial fibrillary acidic protein , chemically defined medium , growth factor , biology , biochemistry , cerebrum , growth medium , chemistry , microbiology and biotechnology , endocrinology , immunology , in vitro , immunohistochemistry , central nervous system , genetics , receptor , bacteria

Astrocytes purified from primary cultures of neonatal rat cerebrum can now be grown in a synthetic medium supplemented with putrescine, prostaglandin F2 alpha, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have a marked synergistic effect on growth when used in combination but have little effect when used individually. Astrocytes grown in the defined medium exhibit dramatic changes in morphological characteristics in comparison to cells grown in serum-free or serum-supplemented medium. In addition, these cells express the astrocyte-specific marker glial fibrillary acidic protein and are estimated by several criteria to be greater than 95% astrocytes.

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