Open AccessLow molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo.
Author(s) -
Susan R. Haynes,
Warren R. Jelinek
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.10.6130
Subject(s) - microbiology and biotechnology , biology , transcription (linguistics) , dna , rna , rnase p , rna polymerase iii , polymerase , chinese hamster , alu element , nucleic acid thermodynamics , genetics , rna dependent rna polymerase , gene , human genome , genome , philosophy , linguistics
An Alu-type dispersed repeat previously identified in a cloned fragment of Chinese hamster DNA [Haynes, S. R., Toomey, T. P., Leinwand, L. & Jelinek, W. R. (1981) Mol. Cell. Biol. 1, 573-583] serves as a template for cell-free transcription of discrete low molecular weight RNAs by RNA polymerase III [RNA nucleotidyltransferase (DNA-directed), EC 2.7.7.6]. A class of analogous RNAs has been isolated from growing Chinese hamster cells by hybridization of total low molecular weight nuclear RNAs to the cloned DNA fragment from which cell-free transcription occurs. Two-dimensional analysis of RNase digestion products of these RNAs suggests that they are transcribed from multiple members of the Alu-type dispersed repeat family.