Open AccessExcision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.
Author(s) -
Carol Egner,
Douglas E. Berg
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.1.459
Subject(s) - transposase , transposable element , transposition (logic) , inverted repeat , insertion sequence , insertion , biology , genetics , dna transposable elements , tn10 , slippage , indel mutation , reversion , nucleotide excision repair , dna , mutation , microbiology and biotechnology , genome , dna repair , gene , computer science , phenotype , artificial intelligence , single nucleotide polymorphism , genotype , structural engineering , engineering
The excision of Tn5 from sites of insertion in the Escherichia coli genome was studied by examining the reversion of lac::Tn5 insertion mutations to lac+. We find that: (i) the frequency of excision depends on the site of Tn5 insertion, (ii) excision occurs efficiently in recA- cells, (iii) excision does not require a Tn5-encoded transposition function, and (iv) efficient excision requires the inverted repeats of Tn5. We propose that excision of Tn5 is similar to the formation of spontaneous deletions and occurs by slippage during DNA synthesis.