Open AccessSolubilization of active opiate receptors.
Author(s) -
William F. Simonds,
Greg Koski,
Richard A. Streaty,
Leonard M. Hjelmeland,
Werner A. Klee
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.8.4623
Subject(s) - solubilization , receptor , cholic acid , stokes radius , chemistry , neuroblastoma , membrane , biochemistry , cell membrane , biophysics , biology , cell culture , bile acid , enzyme , genetics , size exclusion chromatography
Receptors that reversibly bind opiates and opioid peptides have been solubilized from brain and neuroblastoma-glioma hybrid cell NG108-15 membranes. Active receptors are specifically solubilized with a new type of detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, which is a zwitterionic derivative of cholic acid. The solubilized receptor complexes behave as large molecules with a Stokes radius of 70 A and contain protein as an essential constituent.