Open AccessCloning and mapping of BamHi endonuclease fragments of DNA from the transforming B95-8 strain of Epstein-Barr virus.
Author(s) -
James Skare,
Jack L. Strominger
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.7.3860
Subject(s) - bamhi , restriction enzyme , pbr322 , cloning (programming) , microbiology and biotechnology , cloning vector , biology , dna , genome , multiple cloning site , virus , molecular cloning , strain (injury) , genetics , virology , plasmid , recombinant dna , vector (molecular biology) , gene , peptide sequence , anatomy , computer science , programming language
DNA from the B95-8 strain of Epstein-Barr virus was cleaved into 29 different fragments by BamHI endonuclease (EC 3.1.23.6). All of the fragments except the terminal fragments have been inserted into the pBR322 cloning vector and replicated in Escherichia coli. The location of each cloned DNA fragment in the viral genome has been determined, providing a more detailed physical map of the genome than has been available previously.