Open AccessAnaerobic growth of halobacteria.
Author(s) -
Rainer Hartmann,
Hans-Dieter Sickinger,
Dieter Oesterhelt
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.7.3821
Subject(s) - bacteriorhodopsin , mutant , atp synthase , anaerobic exercise , biochemistry , phosphorylation , substrate (aquarium) , biology , substrate level phosphorylation , chemistry , microbiology and biotechnology , enzyme , gene , ecology , physiology , membrane
An energy-transducing pathway in halobacteria is described. Arginine mediates substrate level phosphorylation and allows the cells to grow anaerobically. Bacteriorhodopsin plus light can function as an alternative energy source under these conditions, provided the cells contain the pigment when transferred to the anaerobic environment. Therefore the selection of mutants functionally defective in ATP synthase or bacteriorhodopsin becomes possible.