Open AccessMeasurement of changes in high-energy phosphates in the cardiac cycle using gated 31P nuclear magnetic renonance.
Author(s) -
Eric T. Fossel,
Howard E. Morgan,
Joanne S. Ingwall
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.6.3654
Subject(s) - creatine , phosphate , high energy phosphate , cardiac cycle , inorganic phosphate , pi , phosphocreatine , nuclear magnetic resonance spectroscopy , cardiac function curve , chemistry , nuclear magnetic resonance , biophysics , energy metabolism , medicine , biochemistry , biology , heart failure , physics
Levels of the high-energy phosphate-containing compounds, ATP and creatine phosphate, and of inorganic phosphate (Pi) were measured as a function of position in the cardiac cycle. Measurements were made on isolated, perfused, working rat hearts through the use of gated 31P nuclear magnetic resonance spectroscopy. Levels of ATP and creatine phosphate were found to vary during the cardiac cycle and were maximal at minimal aortic pressure and minimal at maximal aortic pressure. Pi varied inversely with the high-energy phosphates.