Open AccessProteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors
Author(s) -
Joan McPherson,
Eugene W. Nester,
Milton P. Gordon
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.5.2666
Subject(s) - octopine , agrobacterium tumefaciens , ti plasmid , biology , microbiology and biotechnology , dna , plasmid , transfer dna , nicotiana tabacum , hindiii , agrobacterium , biochemistry , gene , transformation (genetics)
In order to detect proteins that may be produced in crown gall tumors as a result of expression of incorporatedAgrobacterium tumefaciens Ti plasmid DNA (T-DNA), we have isolated mRNA complementary to T-DNA and translated this in a protein-synthesizing system derived from wheat germ. mRNA prepared from cultured E1 tumor fromNicotiana tabacum hybridized withHin dIII fragment 1 sequences of T-DNA immobilized on cellulose nitrate filters. Two proteins of 30,000 and 16,500M r were produced when this selected RNA was released and translated. Other tumor lines fromN. tabacum were investigated, and a protein of slightly less than 30,000M r was encoded byHin dIII fragment 1 sequences of 15955/01 tumor. No products were observed for 15955/1 tumor line, which differs from E1/B6-806 and 15955/01 in that it does not produce octopine. mRNA species of each of the tumor lines hybridized toBst I fragment 8 sequences of T-DNA and produced a common protein of 15,000M r . Because this protein is derived from the region of the T-DNA that is conserved in octopine- and nopaline-type crown gall tumors, it may play a role in oncogenicity.