Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells. | Zendy

M. G. K. Jones | Zendy; R A Bosselman | Zendy; Frans A. van der Hoorn | Zendy; Anton Berns | Zendy; Huijuan Fan | Zendy; Inder M. Verma | Zendy

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Identification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells.

Author(s) -

M. G. K. Jones,

R A Bosselman,

Frans A. van der Hoorn,

Anton Berns,

Huijuan Fan,

Inder M. Verma

Publication year - 1980

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.77.5.2651

Subject(s) - ecori , microbiology and biotechnology , biology , restriction enzyme , nuclease , dna , molecular cloning , recombinant dna , restriction fragment , in vitro recombination , endonuclease , cloning (programming) , virology , genetics , gene , peptide sequence , computer science , programming language

When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoRI, a DNA fragment of 14.0 kilobases can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSVsrc). The cellular DNA fragment contains the entire M-MSVsrc specific sequences. The 14.0-kilobase EcoRI DNA fragment was cloned in bacteriophage lambda. The sequence organization of a recombinant clone, lambda . MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping, and electron microscopy. The results indicate that lambda . MTX-1 contains an uninterrupted stretch of 1.0 kilobase similar to that found in the M-MSV genome.

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