Open AccessIdentification and molecular cloning of Moloney mouse sarcoma virus-specific sequences from uninfected mouse cells.
Author(s) -
Molly Morgan Jones,
R A Bosselman,
Frans A. van der Hoorn,
Anton Berns,
Hua Fan,
Inder M. Verma
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.5.2651
Subject(s) - ecori , microbiology and biotechnology , biology , restriction enzyme , nuclease , dna , molecular cloning , recombinant dna , restriction fragment , in vitro recombination , endonuclease , cloning (programming) , virology , genetics , gene , peptide sequence , computer science , programming language
When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoRI, a DNA fragment of 14.0 kilobases can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSVsrc). The cellular DNA fragment contains the entire M-MSVsrc specific sequences. The 14.0-kilobase EcoRI DNA fragment was cloned in bacteriophage lambda. The sequence organization of a recombinant clone, lambda . MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping, and electron microscopy. The results indicate that lambda . MTX-1 contains an uninterrupted stretch of 1.0 kilobase similar to that found in the M-MSV genome.