Open AccessGenes for three Drosophila heat-shock-induced proteins at a single locus.
Author(s) -
Samuel C. Wadsworth,
Elizabeth A. Craig,
Brian J. McCarthy
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.4.2134
Subject(s) - complementary dna , gene , heat shock protein , biology , microbiology and biotechnology , restriction enzyme , dna , drosophila melanogaster , plasmid , locus (genetics) , genetics , endonuclease , bamhi , hspa12a , heat shock
Two plasmids containing cDNA inserts complementary to the messenger RNAs for the 26,000 and 23,000 molecular weight heat-shock-induced proteins from Drosophila melanogaster were identified. Both of these probes hybridized to a site of a major heat-shock-induced puff, 67B. Hybridization by the gel transfer technique with genome DNA that had been cleaved by a restriction endonuclease indicated that the two genes are closely linked. This expectation was validated through isolation of a DNA fragment generated by BamHI endonuclease containing genes for the 26,000 and 23,000 molecular weight heat-shock-induced proteins and also a gene encoding the 27,000 molecular weight heat-shock-induced protein.