Genes for three Drosophila heat-shock-induced proteins at a single locus. | Zendy

Samuel C. Wadsworth | Zendy; Elizabeth A. Craig | Zendy; Brian J. McCarthy | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Genes for three Drosophila heat-shock-induced proteins at a single locus.

Author(s) -

Samuel C. Wadsworth,

Elizabeth A. Craig,

Brian J. McCarthy

Publication year - 1980

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.77.4.2134

Subject(s) - complementary dna , gene , heat shock protein , biology , microbiology and biotechnology , restriction enzyme , dna , drosophila melanogaster , plasmid , locus (genetics) , genetics , endonuclease , bamhi , hspa12a , heat shock

Two plasmids containing cDNA inserts complementary to the messenger RNAs for the 26,000 and 23,000 molecular weight heat-shock-induced proteins from Drosophila melanogaster were identified. Both of these probes hybridized to a site of a major heat-shock-induced puff, 67B. Hybridization by the gel transfer technique with genome DNA that had been cleaved by a restriction endonuclease indicated that the two genes are closely linked. This expectation was validated through isolation of a DNA fragment generated by BamHI endonuclease containing genes for the 26,000 and 23,000 molecular weight heat-shock-induced proteins and also a gene encoding the 27,000 molecular weight heat-shock-induced protein.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research