Open AccessMultivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12.
Author(s) -
Robert P. Lawther,
G. Wesley Hatfield
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.4.1862
Subject(s) - operon , attenuator (electronics) , l arabinose operon , transcription (linguistics) , gal operon , trp operon , escherichia coli , gene , biology , dna , genetics , microbiology and biotechnology , rna , antitermination , physics , linguistics , philosophy , attenuation , optics
The regulatory region for the ilvGEDA operon of Escherichia coli K-12 has been located and characterized. ilv leader RNA transcribed from this region is described, and the DNA sequence of the region is presented. This DNA sequence contains a transcription promoter, a region coding for a 32-amino-acid polypeptide containing multiple isoleucine, valine, and leucine codons, and a transcription termination site preceding the first structural gene. The mutually exclusive secondary structures of the leader RNA have been analyzed. On the basis of these data, a model for the multivalent attenuation of the ilvGEDA operon is proposed.