Open AccessPolyoma large tumor antigen is not required for tumorigenesis mediated by viral DNA.
Author(s) -
Janet Moore,
K. Chowdhury,
María Ángeles Martin,
Mark A. Israel
Publication year - 1980
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.3.1336
Subject(s) - plasmid , biology , recombinant dna , microbiology and biotechnology , in vitro recombination , dna , virology , restriction enzyme , southern blot , ecori , nucleic acid thermodynamics , carcinogenesis , molecular cloning , gene , genetics , complementary dna , base sequence
The arrangement of viral DNA sequences in a hamster cell line derived from a tumor induced by a recombinant plasmid DNA preparation containing the entire polyoma virus genome was examined. In the recombinant plasmid employed, viral DNA sequences specifying the large species of polyoma tumor antigen but not the small and middle tumor antigens were interrupted by the insertion of plasmid DNA at the EcoRI restriction endonuclease site. Blot-hybridization analyses of tumor cell DNA indicated that the "joints" linking viral and plasmid DNAs in the original recombinant plasmid used in animal inoculation had been preserved. Integration into the hamster cell genome had apparently occurred within plasmid DNA sequences. These results indicate that polyoma large tumor antigen is not required for tumorigenesis mediated by viral DNA.
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