Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences. | Zendy

Don J. Katcoff | Zendy; Uri Nudel | Zendy; D. ZevinSonkin | Zendy; Y. Carmon | Zendy; Moshe Shani | Zendy; Hans Lehrach | Zendy; A.-M. Frischauf | Zendy; David Yaffe | Zendy

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Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences.

Author(s) -

Don J. Katcoff,

Uri Nudel,

D. ZevinSonkin,

Y. Carmon,

Moshe Shani,

Hans Lehrach,

A.-M. Frischauf,

David Yaffe

Publication year - 1980

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.77.2.960

Subject(s) - recombinant dna , plasmid , biology , dna , myosin , microbiology and biotechnology , myosin light chain kinase , actin , rna , messenger rna , gene , biochemistry

The construction and partial characterization of recombinant bacterial plasmids carrying DNA sequences that hybridize with rat skeletal muscle actin and a myosin light chain mRNA is described. DNA of one clone hybridizes specifically with the muscle-specific alpha-actin mRNA. Three plasmid clones contain DNA inserts that hybridize with muscle as well as with nonmuscle actin mRNA. A fifth plasmid contains sequences complementary to mRNA coding for myosin light chain 2. DNA of this plasmid hybridizes specifically with RNA extracted from muscle and differentiated muscle cultures but not with RNA extracted from proliferating mononucleated myogenic cells.

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