An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA. | Zendy

Joseph Shlomai | Zendy; A Kornberg | Zendy

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An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA.

Author(s) -

Joseph Shlomai,

A Kornberg

Publication year - 1980

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.77.2.799

Subject(s) - biology , dna replication , ter protein , microbiology and biotechnology , dna , nucleic acid sequence , dna clamp , dna polymerase ii , genetics , eukaryotic dna replication , gene , rna , reverse transcriptase

Protein n', a prepriming DNA replication enzyme of Escherichia coli, is a phi X174 DNA-dependent ATPase. Restriction of phi X174 DNA have led to the identification of a 55-nucleotide fragment that carries the protein n' recognition sequence. Molecular hybridization and sequence analysis have located this sequence within the untranslated region between genes F and G, a map location analogous to that of the unique complementary strand origin of phage G4 DNA. Within the 55-nucleotide fragment is a sequence of 44 nucleotides that forms a stable hairpin structure. This duplex may be the signal for protein n' to initiate the prepriming events that led to the start of phi X174 complementary DNA strand replication.

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