Open AccessCurrent recorded from a cut-open giant axon under voltage clamp.
Author(s) -
Isabel Llano,
Francisco Bezanilla
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.12.7484
Subject(s) - gating , squid giant axon , voltage clamp , squid , biophysics , potassium channel , membrane potential , axon , membrane , current (fluid) , clamp , sodium channel , chemistry , patch clamp , potassium , anatomy , sodium , electrophysiology , neuroscience , biology , physics , computer science , biochemistry , ecology , clamping , organic chemistry , computer vision , thermodynamics
Squid giant axons were cut open and the resulting membrane sheet was positioned in a chamber separating two compartments. Under voltage clamp, normal sodium, potassium, and gating currents could be recorded for several hours. This preparation allows direct access to the internal side of the axonal membrane and should prove useful for recording of ionic currents, gating currents, and channel-induced current fluctuations in the same membrane sheet.