Open AccessCell cycle-specific mutagenesis at the hypoxanthine phosphoribosyltransferase locus in adult rat liver epithelial cells.
Author(s) -
Charles C. L. Tong,
Michael Fazio,
Gary M. Williams
Publication year - 1980
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.12.7377
Subject(s) - mutagenesis , cell cycle , thymidine , microbiology and biotechnology , hypoxanthine guanine phosphoribosyltransferase , biology , cell , biochemistry , cell synchronization , hypoxanthine , dna synthesis , dna , chemistry , mutation , gene , mutant , enzyme
The cell cycle specificity of chemical mutagenesis was studied by use of two cell synchronization techniques, one a nontoxic technique involving serum deprivation and the other a double thymidine block, to obtain rat liver epithelial cells in different phases of the cell cycle to be exposed to chemical mutagens. For both methyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine, there was a cell cycle specificity of chemical mutagenesis, with the most sensitive phase being the period of DNA synthesis.
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