Open AccessTwo interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.
Author(s) -
Jean Weissenbach,
Yuti Chernajovsky,
Menachem Zeevi,
Lester M. Shulman,
Hermona Soreq,
Uri Nir,
David Wallach,
Michel Perricaudet,
Pierre Tiollais,
Michel Revel
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.12.7152
Subject(s) - cycloheximide , microbiology and biotechnology , biology , escherichia coli , interferon , protein biosynthesis , messenger rna , translation (biology) , plasmid , cloning (programming) , in vitro , fibroblast , pbr322 , dna , biochemistry , virology , gene , computer science , programming language
Two mRNA species that produce biologically active interferon were isolated from human fibroblasts and studied by size fractionation and cloning in Escherichia coli plasmid pBR322. The major fibroblast interferon (Hu IFN-beta 1) is coded for by the smaller of the two mRNAs, an 11S species, 900 nucleotides long, which in cell-free systems yields a 20,000 Mr protein. The second interferon mRNA species (Hu IFN-beta 2) is 14S, about 1300 nucleotides long, and codes for another protein of 23,000-26,000 Mr. The two interferon mRNAs do not cross-hybridize. Both are induced by poly(rI.rC), but IFN-beta 2 mRNA is induced to about 10% in cells by cycloheximide treatment alone whereas under these conditions IFN-beta 1 is not induced.