Open AccessIdentification of two Escherichia coli factor Y effector sites near the origins of replication of the plasmids (ColE1 and pBR322.
Author(s) -
S. Lawrence Zipursky,
Kenneth J. Marians
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.11.6521
Subject(s) - cole1 , pbr322 , replication factor c , plasmid , biology , dna replication , escherichia coli , origin of replication , dna polymerase i , microbiology and biotechnology , dna , dna polymerase ii , eukaryotic dna replication , genetics , gene , polymerase chain reaction , reverse transcriptase
The Escherichia coli replication factor Y has been characterized as a phi X174 (+) strand specific DNA-dependent phosphohydrolase. In conjunction with other E. coli replication proteins, factor Y is involved in the formation of heterogeneous primers that are elongated by the E. coli DNA polymerase III elongation machinery. We report here that the heat-denatured DNAs of plasmids pBR322 and ColE1 serve as effectors for the hydrolysis of ATP by factor Y. The DNA sequences of pBR322 responsible for factor Y effector activity have been localized. Two separate regions of the pBR322 chromosome support Y ATPase activity. These sequences are near the replication origin and are located on opposite DNA strands.