Open AccessMolecular cloning of a human histocompatibility antigen cDNA fragment.
Author(s) -
Hidde L. Ploegh,
Harry T. Orr,
Jack L. Strominger
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.10.6081
Subject(s) - complementary dna , microbiology and biotechnology , biology , untranslated region , nucleic acid sequence , messenger rna , human leukocyte antigen , major histocompatibility complex , reverse transcriptase , molecular cloning , nucleotide , peptide sequence , antigen , chemistry , genetics , dna , gene , polymerase chain reaction
A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was established by its ability to hybridize to HLA heavy chain mRNA and by nucleotide sequence analysis. The pHLA-1 cDNA insert (approximately 525 base pairs) corresponds to the COOH-terminal 46 amino acids of an HLA-A, -B, or -C antigen (15 residues from the hydrophobic region and the remainder from the COOH-termial hydrophilic region), together with a portion of the 3' untranslated region of the mRNA.