Open AccessMethod for preparing cultures of central neurons: cytochemical and immunochemical studies.
Author(s) -
Julio Sotelo,
C. J. Gibbs,
D. Carleton Gajdusek,
Ban Hock Toh,
Marvin Wurth
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.1.653
Subject(s) - central nervous system , biology , microbiology and biotechnology , population , protoplasm , staining , glial fibrillary acidic protein , cell type , astrocyte , cell , neuroscience , cytoplasm , immunohistochemistry , immunology , biochemistry , genetics , demography , sociology
We report a simplified method for culturing fetal central nervous system cells predominantly inducing neurons that grow, differentiate, and live in vitro for as long as 10 weeks. These central nervous system cells form a confluent cell culture in which about 80% of the cells are fully differentiated neurons producing interconnecting axons and dendrite processes and live upon a sparse underlying population of fibrillary and protoplasmic astrocytes, oligodendrocytes, and fibroblasts. Morphological and cytochemical characteristics of these cell types, based on immunofluorescent cell specific markers and silver staining of neurons, are presented.