Biological detection of specific mRNA molecules by microinjection. | Zendy

C. P. Liu | Zendy; Doris L. Slate | Zendy; R A Gravel | Zendy; F.H. Ruddle | Zendy

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Biological detection of specific mRNA molecules by microinjection.

Author(s) -

C. P. Liu,

Doris L. Slate,

R A Gravel,

F.H. Ruddle

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.9.4503

Subject(s) - messenger rna , hypoxanthine phosphoribosyltransferase , microbiology and biotechnology , biology , microinjection , translation (biology) , thymidine kinase , interferon , biochemistry , chemistry , gene , hypoxanthine guanine phosphoribosyltransferase , genetics , mutant , virus , herpes simplex virus

We have used direct microinjection of messenger RNA into individual mouse and human cells to assay for specific translation products. We have been able to detect the synthesis of human fibroblast interferon, thymidine, kinase, hypoxanthine phosphoribosyltransferase, adenine phosphoribosyltransferase, and propionyl-CoA carboxylase in response to injected mRNA. Using the interferon system as a model, we have quantitated interferon synthesis and followed partial purification of interferon mRNA sequences on sucrose density gradients. The methods we have utilized should be applicable to other systems in which sensitive assays exist for gene products and should provide a screening procedure for isolating specific mRNA sequences.

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