Fluorescent phallotoxin, a tool for the visualization of cellular actin. | Zendy

Elisabeth Wulf | Zendy; Axel Deboben | Zendy; Friedlinde A. Bautz | Zendy; Heinz Faulstich | Zendy; Thomas Wieland | Zendy

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Fluorescent phallotoxin, a tool for the visualization of cellular actin.

Author(s) -

Elisabeth Wulf,

Axel Deboben,

Friedlinde A. Bautz,

Heinz Faulstich,

Thomas Wieland

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.9.4498

Subject(s) - phalloidin , actin , microfilament , fluorescence , biophysics , microbiology and biotechnology , biology , podosome , biochemistry , cytoskeleton , cell , physics , quantum mechanics

A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.

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