Open AccessEffect of anti-HLA antisera on macrophage-T-cell interactions.
Author(s) -
Raif S. Geha,
Henry Milgrom,
Martin D. Broff,
Susan Alpert,
S. Martín,
Edmond J. Yunis
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.8.4038
Subject(s) - antiserum , antigen , toxoid , macrophage , biology , human leukocyte antigen , t cell , microbiology and biotechnology , immunology , immune system , immunization , in vitro , biochemistry
Human T lymphocytes were shown to proliferate in response to tetanus toxoid antigen only in the presence of macrophages. This response was inhibited by anti-DRw but not by anti-HLA (A and B loci) antisera added to the cultures and by pretreatment of macrophages but not of T cells with anti-DRw antisera and complement. Macrophages pulsed for 18 hr with antigen and then washed were capable of triggering T-cell proliferation. Addition of anti-DRw but not anti-HLA (A and B loci) antisera during the pulse period inhibited the macrophages' ability to trigger T-cell proliferation. The data obtained indicate that human T cells recognize and proliferate in response to antigen presented by the macrophages in association with Ia-like antigens.