Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12. | Zendy

Florence Colbère-Garapin | Zendy; S. Chousterman | Zendy; F Horodniceanu | Zendy; Philippe Kourilsky | Zendy; A. Garapin | Zendy

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Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

Author(s) -

Florence Colbère-Garapin,

S. Chousterman,

F Horodniceanu,

Philippe Kourilsky,

A. Garapin

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.8.3755

Subject(s) - thymidine kinase , microbiology and biotechnology , plasmid , biology , escherichia coli , restriction enzyme , recombinant dna , gene , herpes simplex virus , bamhi , molecular cloning , phosphotransferase , hybrid plasmid , cloning (programming) , virology , virus , complementary dna , biochemistry , computer science , programming language

A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.

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