Characterization of double-stranded ribonucleic acid in tobacco leaves
Author(s) -
M. Ikegami,
H. FraenkelConrat
Publication year - 1979
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.8.3637
Subject(s) - rna , biology , nuclease , double stranded , biochemistry , genetics , chemistry , dna , gene
Double-stranded RNA was isolated from tobacco leaves and characterized in terms of base composition, density, and nuclease resistance. Although its role in the plant's physiology is not clear, evidence was adduced that it is the product of the RNA-dependent RNA polymerase previously shown to occur in this and other plants. The fact that twice as much fully than partially double-stranded RNA appears to be made favors a regulatory role for the double-stranded RNA rather than a transcriptional intermediate role.
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