Open AccessA pseudoduplication in Lycopersicon pimpinellifolium
Author(s) -
Charles M. Rick,
Steven D. Tanksley,
Jon F. Fobes
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.7.3435
Subject(s) - locus (genetics) , allele , gene duplication , lycopersicon , genetics , biology , gene , isozyme , null allele , botany , biochemistry , enzyme
A double-banded variant ofPrx-2 encountered in a single wild plant in a previous survey of the peroxidases ofLycopersicon pimpinellifolium was subjected to genetic analysis. Segregations obtained from crosses of this true-breeding type with various alleles ofPrx-2 yielded anomalous segregations that are not compatible with a single locus model and suggest activity of alleles of a gene at another, independent locus. A critical test was afforded by studying simultaneous segregation for the apparent duplication and for two alleles at the neighboringPrx-3 , a gene which proves to be very tightly linked withPrx-2 . The results of the new test revealed independence between the double-banded type andPrx-3 , hence also between the former andPrx-2 . The most tenable hypothesis stipulates that an independent genemPx2 1 modifies post-translationally the product coded byPrx-2 + to yield two electrophoretically separable isozymes. These results reveal the risk of assuming, without appropriate genetic tests, that gene duplication is necessarily the cause of multiple banding.