Entrapment of a bacterial plasmid in phospholipid vesicles: potential for gene transfer. | Zendy

R.T. Fraley | Zendy; Chester S. Fornari | Zendy; Samuel Kaplan | Zendy

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Entrapment of a bacterial plasmid in phospholipid vesicles: potential for gene transfer.

Author(s) -

R.T. Fraley,

Chester S. Fornari,

Samuel Kaplan

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.7.3348

Subject(s) - pbr322 , plasmid , liposome , transformation (genetics) , dna , escherichia coli , microbiology and biotechnology , chemistry , plasmid preparation , deoxyribonuclease i , incubation , agarose , biochemistry , biology , gene , base sequence

Entrapment of pBR322 DNA within liposomes was demonstrated by (i) its comigration with liposomes on Sepharose 4B columns, (ii) resistance of its biological activity to DNase digestion, and (iii) identification of plasmid DNA on agarose gels after lipid extraction. The biological activity of the liposome-entrapped plasmid was determined by transformation assays. The incubation of intact liposomes, containing entrapped pBR322, with competent Escherichia coli cells in the standard transformation mixture resulted in the appearance of tetracycline-resistant colonies at a frequency of 1% of the control frequency. Importantly, this frequency was unaffected by the addition of DNase to the incubation mixture, whereas transformation by free pBR322 DNA was totally eliminated after treatment with DNase.

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