Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase. | Zendy

Huilin Yang | Zendy; Knut J. Heller | Zendy; Martin Gellert | Zendy; Geoffrey Zubay | Zendy

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Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.

Author(s) -

Huilin Yang,

Knut J. Heller,

Martin Gellert,

Geoffrey Zubay

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.7.3304

Subject(s) - dna gyrase , dna supercoil , operon , novobiocin , biology , plasmid , gene , transcription (linguistics) , colicin , dna , microbiology and biotechnology , promoter , genetics , circular bacterial chromosome , gene expression , escherichia coli , dna replication , bacteria , linguistics , philosophy

We have used the antibiotics coumermycin A1, novobiocin, and oxolinic acid, which are specific inhibitors of DNA gyrase, to study the coupled transcription and translation of several bacterial and plasmid genes in a DNA-directed cell-free system. The expression of different genes is reduced to different extents by inhibition of DNA gyrase activity. Among the genes tested, the lac operon, an rRNA gene, and the colicin gene of colicin E1 plasmid were found to be most sensitive, while the trp operon and some other genes in colicin E1 plasmid were relatively unaffected by the inhibitors. These results, together with earlier work on the transcription of circular DNA templates, indicate that DNA supercoiling can significantly enhance transcription from certain promoters.

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