Open AccessThe ovalbumin gene: cloning and molecular organization of the entire natural gene.
Author(s) -
Achilles Dugaiczyk,
Savio L. C. Woo,
Donald A. Colbert,
Eugene C. Lai,
Myles L. Mace,
Bert W. O’Malley
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.5.2253
Subject(s) - ovalbumin , gene , biology , dna , molecular cloning , cloning (programming) , microbiology and biotechnology , genetics , genomic library , clone (java method) , gene family , dna sequencing , sequence analysis , gene expression , peptide sequence , antigen , computer science , programming language
We report the analyses of recently cloned restriction fragments of the natural ovalbumin gene that overlap in part with previously cloned DNA fragments but extend further into the flanking sequences of the gene. These clones now permit us to identify the DNA sequence that codes for the 5' end of ovalbumin mRNA. Based on these and previous results, the molecular organization of the entire ovalbumin gene was established. The entire gene is composed of eight structural DNA sequences separated by seven intervening sequences that are not present in the mature mRNA. In addition, an ovalbumin gene clone has been obtained from a chicken gene library. Analysis of DNA isolated from this particular clone by molecular hybridization and electron microscopic mapping revealed that it contains the entire ovalbumin gene a single segment of DNA and its structure was consistent with that predicted from our physical map constructed from individually cloned fragments of the gene.