Cloning in Escherichia coli and physical structure of hepatitis B virion DNA. | Zendy

Patrick Charnay | Zendy; Christine Pourcel | Zendy; A Louise | Zendy; A. Fritsch | Zendy; Pierre Tiollais | Zendy

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Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.

Author(s) -

Patrick Charnay,

Christine Pourcel,

A Louise,

A. Fritsch,

Pierre Tiollais

Publication year - 1979

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.76.5.2222

Subject(s) - ecori , bamhi , restriction enzyme , restriction map , escherichia coli , biology , restriction site , dna , genetics , microbiology and biotechnology , cloning (programming) , restriction fragment , genome , hindiii , plasmid , gene , computer science , programming language

A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.

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