Open AccessCloning in Escherichia coli and physical structure of hepatitis B virion DNA.
Author(s) -
Patrick Charnay,
Christine Pourcel,
Anne Louise,
A. Fritsch,
Pierre Tiollais
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.5.2222
Subject(s) - ecori , bamhi , restriction enzyme , restriction map , escherichia coli , biology , restriction site , dna , genetics , microbiology and biotechnology , cloning (programming) , restriction fragment , genome , hindiii , plasmid , gene , computer science , programming language
A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.